Abstract
Anaerobic ammonium oxidation (anammox) receives considerable attentions from researchers because it could achieve biological denitrification from wastewater with low carbon resource input. During anammox, hydrazine oxidoreductase (HZO) plays a critical role in the biological conversion of ammonium (NH4+) to dinitrogen gas. In this study, we constructed a HZO gene recombinant bacterium by genetic process technology with the aim to effectively remove N2H4 in anammox process. The result indicates about 80% N2H4-N could be removed by the obtained HZO gene recombinant bacterium under the optimum condition (an initial N2H4-N concentration of 5 mg/L and a bacterial dosage of 20 mL). More importantly, the high N2H4-N removal efficiency (about 70%) could be maintained when HZO gene recombinant bacterium spread to the sixth generation. The recombinant protein of HZO gene recombinant bacterium could be successfully expressed and show a stable activity to N2H4 removal under low intensity wastewater, suggesting the removal of N2H4 was attributed to the successful expression of HZO enzyme of anammox bacterium in E. Coli.
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