Abstract
To characterize neutralizing mimotopes, phages were selected from a 12-mer phage display library using three anti-porcine reproductive and respiratory syndrome virus (PRRSV) neutralizing monoclonal antibodies: (1) A1; (2) A2; and (3) A7. Of these, A2 and A7 recognize the mimotope, P2, which contains the SRHDHIH motif, which has conserved consensus sequences from amino acid positions 156 to 161 in the N-terminal ectodomain of GP3. The artificial multi-epitope gene, mp2, was designed by combining three repeats of the mimotope P2. The resulting sequence was inserted into the swinepox virus (SPV) genome to construct a recombinant swinepox virus (rSPV-mp2). The rSPV-mp2 was able to stably express the multi-epitope peptide, mP2, in vitro. The rSPV-mp2 immunized pigs exhibited a significantly shorter fever duration compared with the wtSPV treated group (P < 0.05). There was an enhanced humoral and cellular immune response, decreased number of PRRSV genomic copies, and a significant reduction in the gross lung pathology (P < 0.05) was observed following PRRSV infection in rSPV-mp2-immunized animals. The results suggest that the recombinant rSPV-mp2 provided pigs with significant protection against PRRSV infection.
Highlights
To characterize neutralizing mimotopes, phages were selected from a 12-mer phage display library using three anti-porcine reproductive and respiratory syndrome virus (PRRSV) neutralizing monoclonal antibodies: (1) A1; (2) A2; and (3) A7
The role of Neutralizing antibodies (NA) in protection against PRRSV infection was demonstrated in pigs by passive antibody transfer[10]
Epitope-based vaccines (EVs) are another alternative[27], as the immune response evoked towards the EV is directed only towards the relevant epitopes of the pathogen, and may avoid the pathogenic effects associated with the administration of integral antigens[28]
Summary
Phages were selected from a 12-mer phage display library using three anti-porcine reproductive and respiratory syndrome virus (PRRSV) neutralizing monoclonal antibodies: (1) A1; (2) A2; and (3) A7. The resulting sequence was inserted into the swinepox virus (SPV) genome to construct a recombinant swinepox virus (rSPV-mp[2]). The results suggest that the recombinant rSPV-mp[2] provided pigs with significant protection against PRRSV infection. The PRRS epidemic is caused by the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV), and has resulted in huge economic losses to the Chinese pig industry since 20063–7. The peptide epitope mimics (i.e., mimotopes) used as immunogens are a promising tool for vaccine design, and an efficient means of stimulating the antibody response to native antigens, and providing protection against infection in vivo[31]. A recombinant swinepox virus (rSPV-mp2) expressing the multi-epitope peptide for PRRSV was constructed. Our novel construct may have potential as a porcine-based vaccine against PRRSV infection
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