Abstract
Ingredients of food, especially sugar and starch at high-temperature cooking processes could lead to the formation of acrylamide (AA). This chemical is a harmful carcinogen, a neurotoxicant, a reproductive toxicant, and a carcinogen in animal species. However, the detection of acrylamide contamination in food goes unnoticed. In this work, the mouse monoclonal antibody in the form of a single chain variable fragment (scFv) specific to acrylamide was selected from the murine scfv (muscfv) phage-displayed library. Acrylamide (AA) was used as an antigen for bio-panning. The murine single-chain variable fragment (MuscFv) antibody specific to acrylamide in coffee was successfully constructed, which was determined by ELISA and HPLC. Currently, this is the first study, which describes the selection of antibodies against acrylamide from the muscfv phage-displayed library and could be used as a tool for the detection of acrylamide in coffee.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
