Abstract
Ralstonia solanacearum (R. solanacearum) bacterial wilt is a serious soil borne disease that has caused severe losses in tobacco production. In this study, a homogeneous electrochemical strategy based on the sequence of the RipTALI-9 gene is developed for the first time to sensitively detect R. solanacearum in tobacco. In virtue of the Exo III-aided cyclic signal amplification, the biosensor can sensitively monitor R. solanacearum gene, exhibiting the limit of detection (LOD) is as low as 9.53 ng·mL–1. The developed strategy completely has no need of DNA modification onto electrode surfaces and thus the detection shows high stability. Importantly, this biosensor also demonstrates excellent detection performance in real samples. In artificially inoculated tobacco samples, the LOD is 10.66 ng·mL–1, and in artificially inoculated soil sample, the detectable concentration is as low as 6.25 × 103 CFU·mL–1. These results show that the developed electrochemical assay has potential as an effective R. solanacearum diagnostic tool.
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