Abstract
Background: KSHV (HHV-8) has been detected by PCR in Classical Kaposi's sarcoma (CKS), African KS and AIDS-KS. It seems that this virus plays in important role in the pathogenesis of KS. The present study is to 1) determine whether HHV-8 could be detected in KS lesions found in HIV-1 negative patients on iatrogenic immunosuppressive therapy; 2) whether the DNA sequences of HHV-8 vary in the different forms of KS. Methods: 20 KS tumor specimens were obtained from various patients with KS including renal transplant recipient: 1; rheumatoid arthritis 2; CKS: 6 (3 of them are females); AIDS-KS: 5. DNAs were extracted and PCR was performed using HHV-8 specific primers derived from ORF 26, 38 and 39. The PCR products were further analyzed by directly sequencing. Results: HHV-8 was detected in HIV-negative, iatrogenically, immuno-suppressed patients (renal transplant recipient and rheumatoid arthritis). In addition to CKS. AIDS-KS and African KS, DNA sequencing analysis of three different ORF showed high conservation of HHV-8. A few nuclei acid alterations were detected in ORF 26, 38; however, no nucleic acid alterations were found in ORF 39. Conclusion: Our results indicate that HHV-8 was detected not only in AIDS-KS, CKS and African KS but also in KS lesions from iatrogenically immunosuppressed patients. Furthermore, our data suggest that this DNA sequences of HHV-8 is highly conserved in various forms of KS.
Published Version
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