Abstract
Hybridbio/‐synthetic sensory conjugated polymer nanoparticles (CPNs) are developed for selective label‐free detection of target ssDNA in serum. Carboxylic acid‐functionalized anionic polyfluorene nanoparticles are rationally designed as signal amplifying unit to bioconjugate with amine functionalized single stranded oligonucleotides as a receptor. The covalent DNA coating can significantly improve the photostability of the DNA‐bioconjugated CPNs over a wide range of buffer conditions. Better ssDNA discrimination for the DNA‐bioconjugated CPNs sensor is achieved owing to increased interchain interactions and more efficient exciton transport in nanoparticles. The distinguishable fluorescent color for DNA‐bioconjugated CPNs in the presence of target ssDNA allows naked‐eye detection of ssDNA under UV irradiation.
Highlights
Hybridbio/-synthetic sensory conjugated polymer nanoparticles (CPNs) are proteins.[7,8,9,10,11] Label-free detection of target developed for selective label-free detection of target ssDNA in serum
PF-COOH CPNs treated with aminated DNAP-NH2 and PF-COOH CPNs treated directly with EDC in the absence of DNAP-NH2 were used as control as shown in lane 2 and lane 3
To overcome non-specific biological interactions between the cessful surface bioconjugation of PF-COOH CPNs with oligopolycationic conjugated polymers and charged biomolecules, nucleotides since the DNA bioconjugated nanoparticles have the anionic carboxylic acid-functionalized polyfluorenes
Summary
Hybridbio/-synthetic sensory conjugated polymer nanoparticles (CPNs) are proteins.[7,8,9,10,11] Label-free detection of target developed for selective label-free detection of target ssDNA in serum. CPEs to detect anionic DNA and the CPNs sensors can improve functionalized PF-COOH CPNs. The oligonucleotide probe detection sensitivity due to increased interchain interactions and more efficient exciton transport in nanoparticles.
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