Abstract
Conjugated cis, trans- (trans, cis) octadecadienoic acids of milk fat were isolated by preparative gas liquid chromatography and preparative thin layer chromatography of their methyl esters. Reductive ozonolysis and partial hydrazine reduction followed by reductive ozonolysis of resulting cis- and trans-monoene fractions showed that the conjugated cis, trans- (trans, cis) octadecadienoic acids of milk fat consisted essentially of cis-9, trans-11-octadecadienoic acid. Gas liquid chromatography fatty acid profiles with 50-m wall-coated open tubular columns showed components having the same equivalent chain length as conjugated trans, trans-octadecadienoic acid and cis, cis-octadecadienoic acid.
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