Abstract
Identification of DNA conformations in delivery cargoes is important to understand the transfection mechanisms. Conformations of poly(dG-dC)•poly(dG-dC), as a model of DNA, were studied in two different cationic liposome complexes prepared from N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA) and N,NI,NII,NIII-tetramethyl-N,NI,NII,NIII-tetrapalmithyl spermine (TM-TPS) with a helper lipid, dioleoyl phosphotidylethanolamine (DOPE) by Raman microscopy. Characteristic spectral changes in guanine peaks and cytosine peaks of the poly(dG-dC)•poly(dG-dC) distinguished between the right-handed B-form and left-handed Z-form inside the cationic liposome complexes. The poly(dG-dC)•poly(dG-dC) in the TM-TPS/DOPE liposome undergoes the B−Z transition while no conformation change was observed in the poly(dG-dC)•poly(dG-dC) inside the DOTMA/DOPE liposome. Each multi-charged TM-TPS molecule contains four cationic nitrogen atoms in the headgroup. The DOTMA molecule with less cationic nitrogens does not...
Published Version
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