Abstract

The axial beam scanning in confocal microscopy has been implemented using liquid crystal spatial light modulator (LC-SLM). A zoom illuminating lens is realized by introducing LC-SLM into the confocal illumination light path, and thus it shifts the focus of the objective lens axially. The axial optical sectioning in a conventional confocal microscope is hereby achieved by beam scanning rather than mechanically moving the objective lens. The axial focus shift of the objective lens is realized by changing the modulation phase bitmaps of LC-SLM. By electrically controlling LC-SLM, it has been demonstrated that the focus can shift in the axial direction continuously over a 100μm range, while the FWHM of the focal spot keeps the same as that without LC-SLM. Experimental results further show that the axial intensity curves shift axially in confocal microscopy and confocal axial beam scanning replacing mechanical scanning can therefore be implemented.

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