Abstract

Conference Proceedings \u2013 4th International Conference on Molecular Diagnostics and Biomarker Discovery: Antibody Technology

Highlights

  • Cancer is responsible for increase in the mortality rate and has become a life threatening disease affecting people at all ages in both developing and developed countries

  • There were 5 groups in this study, namely: (CN) normal pregnant rat; (PC) mild-preeclamptic rat; (P1) preeclamptic rat treated with nifedipine; (P2) preeclamptic rat treated with anthocyanin, (P3) preeclamptic rat treated with a combination of nifedipine and anthocyanin

  • It was coated in microwell plates to conduct Enzyme Linked Immunosorbent Assay (ELISA) in order to perform immunogenicity that can bind with anti-thyroglobulin that contains in sera

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Summary

Introduction

Cancer is responsible for increase in the mortality rate and has become a life threatening disease affecting people at all ages in both developing and developed countries. The present study aims to (i) identify miR-100 associated target genes; (ii) to perform functional enrichment analysis of miR-100 identified target genes and (iii) to identify experimentally validated miR-100 target genes in human cancers so as to decipher the potential molecular mechanisms regulated by miR-100 in APL. Based on the MAT status, the sensitivity and specificity of the developed ELISA was 90.9% and 90%, respectively This finding showed that infected patients produced a detectable amount of anti-LipL21 IgG/IgM antibodies. Based on this serological result, it is assumed that the expressed recombinant protein fold correctly, similar to its native counterpart [1]. This study was aimed to develop a method of ssDNA production using asymmetric PCR coupled with a streptavidin-conjugated resin that acts as dsDNA capturing agent

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