Abstract
A high flux, thin film composite reverse osmosis (RO) membrane was used to concentrate L-phenylalanine (L-Phe) from clarified bioreactor harvest media. At pH 10±0.5 and 50°C, concentrations of 100 g l −1 were easily achieved and at fluxes from 17 to 119 1 m −2 h −1. Rejection coefficient for L-Phe was inversely proportional (as the log) to retentate concentration. A preliminary system study showed that stages in a cascade could be used to recover essentially all of the product from clarified harvests. The study shows the importance of empirical evaluation as the basis of design and suggests that bioprocess applications of RO are likely to be case specific.
Published Version
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