Computer-assisted retention prediction system for inorganic cyclic polyphosphates and its application to optimization of gradients in anion-exchange chromatography

Abstract

A computer-assisted technique for the retention prediction of inorganic cyclic polyphosphates under gradient elution conditions in anion-exchange chromatography is presented. A computer-assisted retention prediction system, which is an alternative to high-performance liquid chromatographic computer simulation systems, finds an optimum gradient easily in the anion-exchange separations of cyclic polyphosphates. The effect of gradient on the resolution and band spacing is illustrated by computer simulation. An increase in the initial salt concentration of the eluent is the most effective factor for the complete resolution of cyclic polyphospates. A convex gradient is also essential for optimum resolution.