Abstract
A computer-assisted retention prediction system which is an alternative to computer simulation is demonstrated to be an efficient tool for finding optimum gradients in anion-exchange separations of oligonucleotides. The effect of the gradient profile on the resolution is illustrated by computer simulation in the visualization of simulated chromatograms by changing the variables for the gradient profile. A convex gradient was found to be more effective for the separation of high-molecular-weight oligonucleotidas than a linear gradient which has been widely used. An increase in the gradient time also improved the resolution. Recommendations are presented for an approach to maximize the resolution and to minimize the analysis time in the anion-exchange high-performance liquid chromatography of olignonucleotides.
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