Comprehensive screening of polybromochlorodibenzo-p-dioxins, dibenzofurans as mixed halogenated compounds in wastewater samples from industrial facilities by GC×GC/ToFMS and post-data processing

Abstract

An enormous number of pollutants must be investigated to be able to understand which types threaten human health and environmental biota. In this study, we propose a workflow for screening polybromochlorodibenzo-p-dioxins and dibenzofurans (PBCDD/Fs), which are compounds that have thousands of isomers and congeners, by combining measurement of a sample without any in-laboratory-cleanup with the results of comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry and post-data processing. This process can be regarded as “in silico sample cleanup.” The post-data processing stage comprises two methods in which the extracted mass spectra are matched to exact mass and isotopic ratios specified as formulae and filtering via mass deficiency. We applied this workflow to wastewater samples from industrial facilities to identify mixtures of halogenated dioxins. As a result, it was estimated that dioxins in an absolute quantity of 10–500 pg could be detected with sufficient accuracy by recovery testing of a standard mixture against sample crude extracts. Tri- to octa-halogenated dioxins were detected in 8 of 13 samples. Leachate from an industrial landfill was found to contain relatively large numbers of PBCDD/Fs, and several congeners were found in wastewater from an industrial fabric facility that handles decabromodiphenyl ether.The workflow, including the post-data processing method developed and applied in this study, has the advantage that additional identifications can be performed at any time from a single set of measurement data. This also enables the screening of substances that have thousands of homologous isomers, such as chlorinated and brominated dioxins, as well as other non-halogenated compounds.