Abstract
ObjectiveGraves’ disease (GD) related hyperthyroidism (HT) has profound effects on metabolic activity and metabolism of macromolecules affecting energy homeostasis. In this study, we aimed to get a comprehensive understanding of the metabolic changes and their clinical relevance in GD children.MethodsWe investigated serum substances from 30 newly diagnosed GD children and 30 age- and gender-matched healthy controls. We explored the metabolomics using ultra-high-performance liquid chromatography–quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) analysis, and then analyzed the metabolomic data via multivariate statistical analysis.ResultsBy untargeted metabolomic analysis, a total of 730 metabolites were identified in all participants, among which 48 differential metabolites between GD and control groups were filtered out, including amino acids, dipeptides, lipids, purines, etc. Among these metabolites, 33 were detected with higher levels, while 15 with lower levels in GD group compared to controls. Pathway analysis showed that HT had a significant impact on aminoacyl-transfer ribonucleic acid (tRNA) biosynthesis, several amino acids metabolism, purine metabolism, and pyrimidine metabolism.ConclusionIn this study, via untargeted metabolomics analysis, significant variations of serum metabolomic patterns were detected in GD children.
Highlights
Graves’ disease (GD) is the most common cause of hyperthyroidism (HT) with an autoimmune origin in children and adults [1,2,3]
Metabolic pathways, such as arginine and proline metabolism and aminoacyl-transfer ribonucleic acid biosynthesis have been altered in GD patients [15]
We used an ultra-high-performance liquid chromatography coupled with the quadruple time-offight mass spectrometry (UHPLC-QTOF/MS)-based untargeted metabolomics approach to explore the perturbation of metabolic process in GD children compared to age- and gender- matched healthy normal controls
Summary
Graves’ disease (GD) related hyperthyroidism (HT) has profound effects on metabolic activity and metabolism of macromolecules affecting energy homeostasis. We aimed to get a comprehensive understanding of the metabolic changes and their clinical relevance in GD children
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