Abstract
The anther culture technique, although it has been widely used in wheat breeding programs, is associated with a high albinism rate that limits its application. The mechanism of albinism has not been studied explicitly at the molecular level. In this study, differently expressed genes (DEGs) and proteins (DEPs) of albino and green seedlings from a wheat anther culture were analyzed and compared using transcriptomic sequencing (RNA-Seq), two-dimensional electrophoresis, and mass spectrometry. A total of 1 892 DEGs (1 115 down-regulated and 777 up-regulated) and 43 DEPs (15 down-regulated and 28 up-regulated) that are primarily involved in photosynthetic pathways and porphyrin and chlorophyll metabolism was identified when the albino group library was used as the control. Most DEGs participated in constructing components of thylakoids and the chloroplast envelope as determined via gene ontology analysis. The expression patterns of 12 selected genes were evaluated using real-time quantitative PCR, and the results were in agreement with the RNA-Seq analysis. Our results may assist the development of new methods to decrease the albino seedling rates in wheat anther cultures.
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