Abstract
BackgroundZinc finger proteins (ZFPs) containing only a single zinc finger domain play important roles in the regulation of plant growth and development, as well as in biotic and abiotic stress responses. To date, the evolutionary history and functions of the ZFP gene family have not been identified in cotton.ResultsIn this paper, we identified 29 ZFP genes in Gossypium hirsutum. This gene family was divided into seven subfamilies, 22 of which were distributed over 17 chromosomes. Bioinformatic analysis revealed that 20 GhZFP genes originated from whole genome duplications and two originated from dispersed duplication events, indicating that whole genome duplication is the main force in the expansion of the GhZFP gene family. Most GhZFP8 subfamily genes, except for GhZFP8–3, were highly expressed during fiber cell growth, and were induced by brassinosteroids in vitro. Furthermore, we found that a large number of GhZFP genes contained gibberellic acid responsive elements, auxin responsive elements, and E-box elements in their promoter regions. Exogenous application of these hormones significantly stimulated the expression of these genes.ConclusionsOur findings reveal that GhZFP8 genes are involved in cotton fiber development and widely induced by auxin, gibberellin and BR, which provides a foundation for the identification of more downstream genes with potential roles in phytohormone stimuli, and a basis for breeding better cotton varieties in the future.
Highlights
Zinc finger proteins (ZFPs) containing only a single zinc finger domain play important roles in the regulation of plant growth and development, as well as in biotic and abiotic stress responses
Arabidopsis ZFP protein sequences were used as queries to search the three reference genomes to screen out candidate ZFP proteins in cotton
Using HMMR software for further selection of ZFP candidates based on conserved domains, we identified 29 ZFPs in G. hirsutum, along with 23 in G. arboreum and 23 in G. raimondii
Summary
Zinc finger proteins (ZFPs) containing only a single zinc finger domain play important roles in the regulation of plant growth and development, as well as in biotic and abiotic stress responses. Exogenous application of GA3 improved fiber elongation, increased fiber cell wall thickness. He et al BMC Plant Biology (2019) 19:329 and increased the weight of individual fibers [3, 4]. Exogenous application of indole-3-acetic acid (IAA) significantly increased the total fiber volume [8], while application of IAA transport inhibitor, 1-naphthylphthalamic acid (NPA), dramatically reduced IAA content and the number of fiber cells [9]. Overexpression of the IAA biosynthetic gene iaaM significantly increased fiber cell number, final yield, and overall quality [9]
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