Abstract
Combining the scattered light of gold nanoparticles (GNPs) and the fluorescence of dye molecules, a compound cellular imaging of laser scanning confocal microscopy (LSCM) is obtained. The human breast cancer cell line (MDA-MB-435S, BCRC 60429) is used for experiment. These cells are incubated with a glucose medium containing GNPs for 26 hours, and then are stained by Prodium Iodide (PI) for their nuclei. By using a single laser to illuminate these cells and adjusting the ranges of two bandpass filters for the detection, the scattered light from the GNPs and the fluorescence of PI can be induced simultaneously, but be detected separately without crosstalk. Furthermore, a compound cellular image can be obtained by merging the two images of the expressions of GNP and PI together. From the TEM images of these cells, it is observed that GNPs are aggregated in the vesicles of the cytoplasm due to the cell's endocytosis. The aggregation of GNPs makes the surface plasmon resonance band of GNPs broadened, so that strong scattered light from GNPs can be generated by the illumination of different-wavelength lasers (458, 488, 514, 561, and 633 nm).
Highlights
The use of fluorescent dyes and fluorescent proteins as biomarkers becomes an important technique for cellular imaging of laser scanning confocal microscopy (LSCM) recently
Due to the surface plasmon resonance (SPR) of metallic nanoparticles (MNPs), which is a collective oscillation of electrons in the metal, a strong light scattering from MNPs can be induced when MNPs are irradiated by a light within a certain range of ultraviolet (UV) to near-infrared (NIR)
We found that normally the higher the optical density (OD) value, the higher the expression of gold nanoparticles (GNPs) in the cellular image
Summary
The use of fluorescent dyes and fluorescent proteins as biomarkers becomes an important technique for cellular imaging of laser scanning confocal microscopy (LSCM) recently. A variety of dye molecules or proteins with different excitation and emission spectra were developed in the past decades. Combining different kinds of fluorophores and fluorescent proteins for staining the cells, a multi-fluorescence image of LSCM can be obtained by irradiating the sample with differentwavelength lasers. The other new biomarkers [1], e.g., quantum dots (QDs) [2, 3] and metallic nanoparticles (MNPs) [4, 5], are developed for cellular imaging recently. By using different-wavelength lasers (458, 488, 514, 561, and 633 nm) individually, strong scattered lights from GNPs and fluorescence of PI can be induced simultaneously. Dye PI can only be induced by the exciting laser with a wavelength shorter than 600 nm, so that no fluorescence of PI is detected by using laser of 633 nm
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
