Study on Lidocaine Inhibiting Proliferation, Migration and Invasion of Breast Cancer Cells by Modulating the MicroRNA-495-3p/Fibroblast Growth Factor 9 Axis

Abstract

To investigate the mechanism of lidocaine in inhibiting proliferation, migration and invasion of breast cancer cells. Human breast cancer cell line Michigan Cancer Foundation-7 cells were treated with lidocaine. Cell proliferation was detected by 2,5-diphenyl-2H-tetrazolium bromide, cell migration and invasion was detected by Transwell, microRNA-495-3p expression was detected by quantitative reverse transcription polymerase chain reaction and fibroblast growth factor 9, cyclin D1, matrix metalloproteinase 2 and matrix metalloproteinase 9 protein expression were detected by western blot. Human breast cancer cell line Michigan Cancer Foundation-7 cells were transfected with microRNA-495-3p inhibitor or fibroblast growth factor 9 overexpression vector. The effects of silencing microRNA-495-3p or fibroblast growth factor 9 overexpression on proliferation, migration and invasion of human breast cancer cell line Michigan Cancer Foundation-7 cells and the expression of cyclin D1, matrix metalloproteinase 2 and matrix metalloproteinase 9 protein were detected by 2,5-diphenyl-2H-tetrazolium bromide, Transwell and western blot. The dual-luciferase reporter gene experiment confirmed the relationship between microRNA-495-3p and fibroblast growth factor 9 targeting. Lidocaine could inhibit the proliferation, migration and invasion of human breast cancer cell line Michigan Cancer Foundation-7 cells, promote the expression of microRNA- 495-3p and inhibit the expression of fibroblast growth factor 9 protein in human breast cancer cell line Michigan Cancer Foundation-7 cells. Silencing microRNA-495-3p expression or fibroblast growth factor 9 overexpression decreased the inhibitory effect of lidocaine on proliferation, migration and invasion of human breast cancer cell line Michigan Cancer Foundation-7 cells. MicroRNA-495-3p negatively regulated fibroblast growth factor 9 expression in human breast cancer cell line Michigan Cancer Foundation-7 cells. Silencing fibroblast growth factor 9 expression reversed the effect of microRNA-495-3p silencing on proliferation, migration and invasion of human breast cancer cell line Michigan Cancer Foundation-7 cells treated with lidocaine. Lidocaine inhibits the expression of fibroblast growth factor 9 by up-regulating microRNA-495-3p, thus inhibiting the proliferation, migration and invasion of breast cancer cells.