Abstract
The oligosaccharide κ-carrageenan is sulfated carbohydrate with various bioactivities. In order to prepare this functional oligosaccharide directly from the red alga Kappaphycus striatum, a continuous enzymatic hydrolysis method was used to digest K. striatum. The yield of oligosaccharide (product KS-3) reached 43.26 g per 100 g of dry algae, and the product was identified as high-purity sulfated oligosaccharides mainly composed of κ-carrageenan hexasaccharide ([A-G4S]3) and octasaccharide ([A-G4S]4). The by-products generated during the preparation of oligosaccharide were also collected. The yield of insoluble product (KS-1) was 14.46 g per 100 g of dry algae, it was rich in protein (63.23%) and the content of essential amino acid was close to 40 g per 100 g of dry protein. The yield of soluble high-molecular weight product (KS-2) was 18.04 g per 100 g of dry algae, it was mainly composed of soluble dietary fibers (69.49%) and showed perfect expansion characteristics. We describe here a novel method for the preparation of oligosaccharides directly from seaweed, which is also beneficial for the comprehensive utilization of seaweed.
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