Composite of AuNPs@SiO $$_{2}$$ 2 NPs@[(NIPAM)- $${\varvec{b}}$$ b -(Ala)] and its activity on leukemia cells

Abstract

Synthesis and characterization of thermo-sensitive block copolymer of N-isopropyl acryl amide-b-Alanine [(NIPAM)-b-(Alanine)] thin film and its doping with (AuNPs)–(\(\hbox {SiO}_{2}\hbox {NPs}\)), (gold and silica nanoparticles) were reported. Further, composite effect on K562 (leukemia) cells was examined based on in vitro cell-based studies. The synthesis of \(\hbox {SiO}_{2} \hbox {NPs}\) was followed through facile Stober’s sol–gel synthesis methods. The individual morphology of [(NIPAM)-b-(Alanine)] thin film, AuNPs and \(\hbox {SiO}_{2 }\)NPs including [(NIPAM)-b-(Alanine)]@(Au)-(\(\hbox {SiO}_{2} \hbox {NPs}\)) composite was confirmed by using TEM instrumentation. [(NIPAM)-b-(Alanine)] thin film was embedded with gold and silica nanoparticles followed by the sonication. The average size of AuNPs is 16 nm and for \(\hbox {SiO}_{2}\hbox {NPs}\), it is \({\sim }\)368 nm (in diameter). Synthesized composite [(NIPAM)-b-(Alanine)]@(Au)@(\(\hbox {SiO}_{2}\hbox {NPs}\)) is biocompatible for mankind use. However, composite used to examine the inhibitory activity on K562 cells and it shows \(\sim \)78% inhibition, which is significant value for 24 h treatment under humidified atmospheric conditions.