Abstract
Considerable progress in understanding of the mechanism of glycolytic self-oscillation has been made (1) by the elucidation of the input condition necessary for the induction of glycolytic oscillations; (2) by a detailed analysis of the concentration pattern of glycolytic intermediates; (3) by titration experiments with inhibitors, metabolites, and enzymes; (4) by analysis of enzyme activities and enzyme kinetics mainly of phosphofructokinase, phosphoglycerate kinase, and phosphokinase under the conditions of oscillation; and (5) by theoretical studies of oscillating chemical systems. This chapter discusses the concentrations of the glycolytic enzymes and metabolites, and the concentration of the enzyme -coenzyme complexes of the dehydrogenases under oscillating conditions. The application of the Michaelis-Menten equation for enzyme-saturated conditions leads to values for molarities in the extract, or in reconstructed systems.
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