COMPONENT COMPOSITION OF ESSENTIAL OIL THYMUS PULCHELLUS S. A. MEY., INTRODUCED UNDER DAGESTAN CONDITIONS

Abstract

The purpose of the study is to study the component composition of the essential oil in the aerial part of the endemic Krasnodar specimen Thymus pulchellus S. A. Mey., transplanted to the Tsudakhar experimental base of the Mountain Botanical Garden of the Dagestan Federal Research Center of the Russian Academy of Sciences (CEB, 1000 m above sea level, Dagestan, Russia ) to search for plants with a high yield of essential oil with a maximum content of valuable biologically active components and expand the growing area of this type of thyme. Material and methods. The aerial part of the Thymus pulchellus introduced at the CEB was collected in the flowering phase. The essential oil was obtained by hydrodistillation (Clevenger method) for 2 hours from dry raw materials prepared by conventional methods. Component qualitative and quantitative analysis of the isolated essential oil samples was carried out by chromatography-mass spectrometry on a Shimadzu GCMSQP2010plus on a Supelco SLB TM-5 ms column (30 m × 0.25 mm × 0.25 μm) in split mode. Results. The EF yield was 0.26% in terms of air-dry raw materials. Using chromatography-mass spectrometry, 56 components were isolated from EF samples, of which 97.67% of compounds were identified. Conclusions. For the first time, data are presented on the study of the composition of the essential oil from the aerial part of the introduced Dagestan sample of Thymus pulcellus. The resulting essential oil consists mainly of the sesquiterpene fraction (84.12%). Compared to other species, the essential oil of the studied pretty thyme showed a completely different set of main components, among which the main ones are limonene, γ-cadinene, α-muurolol, epicubenol, which allows us to assert the isolation of this species. The results obtained allow us to characterize the essential oil of Thymus pulchellus as a source of valuable components, and the plant itself as a promising medicinal raw material.