Abstract
λ repressor-mediated DNA loop formation and breakdown were monitored by Tethered Particle Microscopy (TPM) (1). The dwell times of the looped and unlooped DNA states, as revealed from TPM traces, were analyzed and revealed a complex kinetics for both loop formation and loop breakdown. A mechanism is proposed were λ repressor non-specific binding to DNA may play an important physiological role. (1) C. Zurla, C. Manzo, D.D. Dunlap, D.E.A. Lewis, S. Adhya, L. Finzi, “Direct demonstration and quantification of long-range DNA looping by the λ bacteriophage repressor.”, NAR, 37, 2789-2795, 2009.
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