Complete mitogenome of Olidiana ritcheriina (Hemiptera: Cicadellidae) and phylogeny of Cicadellidae.

Xian-Yi Wang,Zhi-Hua Fan,Ren-Huai Dai,Jia-Jia Wang

doi:10.7717/peerj.8072

Xian-Yi Wang, Zhi-Hua Fan + Show 2 more

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https://doi.org/10.7717/peerj.8072

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Journal: PeerJ	Publication Date: Nov 26, 2019
Citations: 11	License type: CC BY 4.0

Affiliation: Guizhou University, Tangshan College

Abstract

BackgroundCoelidiinae, a relatively large subfamily within the family Cicadellidae, includes 129 genera and ∼1,300 species distributed worldwide. However, the mitogenomes of only two species (Olidiana sp. and Taharana fasciana) in the subfamily Coelidiinae have been assembled. Here, we report the first complete mitogenome assembly of the genus Olidiana.MethodsSpecimens were collected from Wenxian County (Gansu Province, China) and identified on the basis of their morphology. Mitogenomes were sequenced by next-generation sequencing, following which an NGS template was generated, and this was confirmed using polymerase chain reaction and Sanger sequencing. Phylogenic trees were constructed using maximum likelihood and Bayesian analyses.ResultsThe mitogenome of O. ritcheriina was 15,166 bp long, with an A + T content of 78.0%. Compared with the mitogenome of other Cicadellidae sp., the gene order, gene content, gene size, base composition, and codon usage of protein-coding genes (PCGs) in O. ritcheriina were highly conserved. The standard start codon of all PCGs was ATN and stop codon was TAA or TAG; COII, COIII, and ND4L ended with a single T. All tRNA genes showed the typical cloverleaf secondary structure, except for trnSer, which did not have the dihydrouridine arm. Furthermore, the secondary structures of rRNAs (rrnL and rrnS) in O. ritcheriina were predicted. Overall, five domains and 42 helices were predicted for rrnL (domain III is absent in arthropods), and three structural domains and 27 helices were predicted for rrnS. Maximum likelihood and Bayesian analyses indicated that O. ritcheriina and other Coelidiinae members were clustered into a clade, indicating the relationships among their subfamilies; the main topology was as follows: (Deltocephalinae + ((Coelidiinae + Iassinae) + ((Typhlocybinae + Cicadellinae) + (Idiocerinae + (Treehopper + Megophthalminae))))). The phylogenetic relationships indicated that the molecular taxonomy of O. ritcheriina is consistent with the current morphological classification.

Highlights

Coelidiinae is a relatively large subfamily within the Cicadellidae family, and it includes 129 genera and approximately 1,300 species (Nielson, 2015), including some species thatHow to cite this article Wang X-Y, Wang J-J, Fan Z-H, Dai R-H. 2019
Composition analysis revealed that the mitogenome of O. ritcheriina exhibited a positive AT (0.144) and negative GC skew (−0.227) in the whole mitogenome as well as in the 13 protein-coding genes (PCGs) (AT skew: 0.163; GC skew: −0.250), 2 rRNAs (AT skew: 0.160; GC skew: −0.265), and 22 tRNAs (AT skew: 0.111; GC skew: −0.110)
We sequenced the mitogenome of O. ritcheriina from Coelidiinae and presented their structure and sequence characteristics

Summary

Introduction

Coelidiinae is a relatively large subfamily within the Cicadellidae family, and it includes 129 genera and approximately 1,300 species (Nielson, 2015), including some species thatHow to cite this article Wang X-Y, Wang J-J, Fan Z-H, Dai R-H. 2019. Coelidiinae is a relatively large subfamily within the Cicadellidae family, and it includes 129 genera and approximately 1,300 species (Nielson, 2015), including some species that. Complete mitogenome of Olidiana ritcheriina (Hemiptera: Cicadellidae) and phylogeny of Cicadellidae. Coelidiinae, a relatively large subfamily within the family Cicadellidae, includes 129 genera and ∼1,300 species distributed worldwide. Compared with the mitogenome of other Cicadellidae sp., the gene order, gene content, gene size, base composition, and codon usage of protein-coding genes (PCGs) in O. ritcheriina were highly conserved. Maximum likelihood and Bayesian analyses indicated that O. ritcheriina and other Coelidiinae members were clustered into a clade, indicating the relationships among their subfamilies; the main topology was as follows: (Deltocephalinae + ((Coelidiinae + Iassinae) + ((Typhlocybinae + Cicadellinae) + (Idiocerinae + (Treehopper + Megophthalminae))))). The phylogenetic relationships indicated that the molecular taxonomy of O. ritcheriina is consistent with the current morphological classification

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