Abstract
BackgroundRepresentatives of the trematode family Fasciolidae are responsible for major socio-economic losses worldwide. Fascioloides magna is an important pathogenic liver fluke of wild and domestic ungulates. To date, only a limited number of studies concerning the molecular biology of F. magna exist. Therefore, the objective of the present study was to determine the complete mitochondrial (mt) genome sequence of F. magna, and assess the phylogenetic relationships of this fluke with other trematodes based on the mtDNA dataset.FindingsThe complete F. magna mt genome sequence is 14,047 bp. The gene content and arrangement of the F. magna mt genome is similar to those of Fasciola spp., except that trnE is located between trnG and the only non-coding region in F. magna mt genome. Phylogenetic relationships of F. magna with selected trematodes using Bayesian inference (BI) was reconstructed based on the concatenated amino acid sequences for 12 protein-coding genes, which confirmed that the genus Fascioloides is closely related to the genus Fasciola; the intergeneric differences of amino acid composition between the genera Fascioloides and Fasciola ranged 17.97–18.24 %.ConclusionsThe determination of F. magna mt genome sequence provides a valuable resource for further investigations of the phylogeny of the family Fasciolidae and other trematodes, and represents a useful platform for designing appropriate molecular markers.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1699-7) contains supplementary material, which is available to authorized users.
Highlights
Representatives of the trematode family Fasciolidae are responsible for major socio-economic losses worldwide
The determination of F. magna mt genome sequence provides a valuable resource for further investigations of the phylogeny of the family Fasciolidae and other trematodes, and represents a useful platform for designing appropriate molecular markers
KR006934) is 14,047 bp in length (Fig. 1) and contains 36 genes that are transcribed in the same direction, including 12 protein-coding genes, 22 tRNA genes and two rRNA genes, lacking the atp8 gene (Table 1), consistent with those of selected trematode species available on GenBank [17,18,19, 27, 28]
Summary
Representatives of the trematode family Fasciolidae are responsible for major socio-economic losses worldwide. Fascioloides magna is an important pathogenic liver fluke of wild and domestic ungulates. Fascioloides magna, known as the large American liver fluke, giant liver fluke or deer fluke, is an important digenetic trematode of the family Fasciolidae [3, 4]. Migration of F. magna immature flukes within the host body often leads to profound damage to the liver and other organ tissues [8, 12], causing economic losses worldwide [13]. A sequence of nuclear ribosomal DNA (rDNA) of F. magna was obtained in 2008 [14], partial sequences of mitochondrial (mt) genes, such as cytochrome c oxidase
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