Complete genome sequences of Geobacillus sp. Y412MC52, a xylan-degrading strain isolated from obsidian hot spring in Yellowstone National Park.

Phillip Brumm,Yun-Juan Chang,Miriam L Land,Loren J Hauser,David A Mead,Cynthia D Jeffries

doi:10.1186/s40793-015-0075-0

Abstract

Geobacillus sp. Y412MC52 was isolated from Obsidian Hot Spring, Yellowstone National Park, Montana, USA under permit from the National Park Service. The genome was sequenced, assembled, and annotated by the DOE Joint Genome Institute and deposited at the NCBI in December 2011 (CP002835). Based on 16S rRNA genes and average nucleotide identity, Geobacillus sp. Y412MC52 and the related Geobacillus sp. Y412MC61 appear to be members of a new species of Geobacillus. The genome of Geobacillus sp. Y412MC52 consists of one circular chromosome of 3,628,883 bp, an average G + C content of 52 % and one circular plasmid of 45,057 bp and an average G + C content of 45 %. Y412MC52 possesses arabinan, arabinoglucuronoxylan, and aromatic acid degradation clusters for degradation of hemicellulose from biomass. Transport and utilization clusters are also present for other carbohydrates including starch, cellobiose, and α- and β-galactooligosaccharides.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-015-0075-0) contains supplementary material, which is available to authorized users.

Highlights

Identification of new organisms that produce biomassdegrading enzymes is of considerable interest
Especially those at Yellowstone National Park, have been a source of many new organisms including Thermus aquaticus [4, 5], Thermus brockianus [6], and Acidothermus cellulolyticus [7] that possess enzymes with significant potential in biotechnological applications [8]
Of interest is the isolation of both low G + C (C56-YS93, 43.9 % G + C) and high G + C (Y412MC52 and Y412MC61, 52.3 % G + C) xylanolytic Geobacillus species from the same hot spring sample

Summary

Introduction

Identification of new organisms that produce biomassdegrading enzymes is of considerable interest. Isolated, characterized, and sequenced a number of new enzyme-producing aerobic organisms from Yellowstone hot springs. Geobacillus species were the most common aerobic organisms isolated during the cultivation of most hot springs samples.

Results

Conclusion