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Abstract
Geobacillus thermoglucosidasius C56-YS93 was one of several thermophilic organisms isolated from Obsidian Hot Spring, Yellowstone National Park, Montana, USA under permit from the National Park Service. Comparison of 16 S rRNA sequences confirmed the classification of the strain as a G. thermoglucosidasius species. The genome was sequenced, assembled, and annotated by the DOE Joint Genome Institute and deposited at the NCBI in December 2011 (CP002835). The genome of G. thermoglucosidasius C56-YS93 consists of one circular chromosome of 3,893,306 bp and two circular plasmids of 80,849 and 19,638 bp and an average G + C content of 43.93 %. G. thermoglucosidasius C56-YS93 possesses a xylan degradation cluster not found in the other G. thermoglucosidasius sequenced strains. This cluster appears to be related to the xylan degradation cluster found in G. stearothermophilus. G. thermoglucosidasius C56-YS93 possesses two plasmids not found in the other two strains. One plasmid contains a novel gene cluster coding for proteins involved in proline degradation and metabolism, the other contains a collection of mostly hypothetical proteins.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-015-0031-z) contains supplementary material, which is available to authorized users.
Highlights
Identification of new organisms that produce biomassdegrading enzymes is of considerable interest
While it is possible that G. thermoglucosidasius C56-YS93 was present only as windblown spores in the hot spring [18], there are a number of strong arguments for the growth of this and other Geobacillus species in hot springs
No Geobacillus species were isolated from acidic hot springs located close to the alkaline and neutral springs
Summary
Identification of new organisms that produce biomassdegrading enzymes is of considerable interest. Geobacillus species were the most common aerobic organisms isolated during the cultivation of most hot springs samples. We therefor sequenced a number of these Geobacillus isolates including G. thermoglucosidasius C56-YS93 to identify new enzymes suitable for use in biomass conversion into fuels and chemicals.
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