Complete Genome Sequence of Thermophilic Bacterium Aeribacillus pallidus PI8.

Kyosuke Kita,Kosei Tanaka,Atsushi Ishida,Shu Ishikawa,Ken-Ichi Yoshida,J Cameron Thrash

doi:10.1128/mra.00224-20

Abstract

Here, we report the complete genome sequence of Aeribacillus pallidus PI8, a thermophilic bacterium, isolated from soybean stem extract. The sequence was determined using Illumina and Nanopore sequencers. Bioinformatic analyses of the genome sequence revealed the presence of possible bacteriocin gene clusters.

Highlights

Eribacillus pallidus is a Gram-positive, aerobic, and endospore-forming thermophilic bacterium previously reclassified from the genus Geobacillus (1)
We were interested in an unknown thermophilic bacterium found in a hot water extract of soybean stems harvested from Nakasatsunai, Hokkaido, Japan, and characterized it as a novel strain of A. pallidus as follows
In order to characterize the unknown species forming the white colonies, one white colony was isolated and inoculated into LB liquid medium to grow at 55°C overnight with shaking at 180 rpm

Summary

Introduction

A eribacillus pallidus is a Gram-positive, aerobic, and endospore-forming thermophilic bacterium previously reclassified from the genus Geobacillus (phylum Firmicutes, family Bacillaceae) (1). Eribacillus pallidus is a Gram-positive, aerobic, and endospore-forming thermophilic bacterium previously reclassified from the genus Geobacillus (phylum Firmicutes, family Bacillaceae) (1). We were interested in an unknown thermophilic bacterium found in a hot water extract of soybean stems harvested from Nakasatsunai, Hokkaido, Japan, and characterized it as a novel strain of A. pallidus as follows. In order to characterize the unknown species forming the white colonies, one white colony was isolated and inoculated into LB liquid medium to grow at 55°C overnight with shaking at 180 rpm.

Results

Conclusion