Complete Genome Sequence of the Virulent Klebsiella pneumoniae Phage Geezett Infecting Multidrug-Resistant Clinical Strains

Belinda Loh,Mark A Toleman,Xiaoqing Wang,Prasanth Manohar,Yunsong Yu,Willames M.B.S Martins,Xiaoting Hua,Sebastian Leptihn,Ramesh Nachimuthu,Liwei Zhang,Long Ma,Catherine Putonti

doi:10.1128/mra.00685-21

Abstract

ABSTRACTGeezett was isolated from hospital sewage in Hangzhou, China, and exhibits lytic activity against clinical isolates of the nosocomial pathogen Klebsiella pneumoniae. The bacteriophage is a myovirus and has a double-stranded DNA (dsDNA) genome 50,707 bp long, containing 79 open reading frames (ORFs).

Highlights

Geezett was isolated from hospital sewage in Hangzhou, China, and exhibits lytic activity against clinical isolates of the nosocomial pathogen Klebsiella pneumoniae
According to the World Health Organization, the emergence of K. pneumoniae strains resistant to carbapenems and third-generation cephalosporins represents an urgent need for development of new antimicrobial agents such as therapeutic phages [2, 3]
Phage Geezett was isolated from sewage water obtained from the Sir Run Run Shaw Hospital in Hangzhou, China, using an enrichment culture of the clinical multidrugresistant K. pneumoniae strain GZ-1

Summary

Introduction

Geezett was isolated from hospital sewage in Hangzhou, China, and exhibits lytic activity against clinical isolates of the nosocomial pathogen Klebsiella pneumoniae. The bacteriophage is a myovirus and has a double-stranded DNA (dsDNA) genome 50,707 bp long, containing 79 open reading frames (ORFs). According to the World Health Organization, the emergence of K. pneumoniae strains resistant to carbapenems and third-generation cephalosporins represents an urgent need for development of new antimicrobial agents such as therapeutic phages [2, 3].

Results

Conclusion