Abstract
ABSTRACTThe soil bacterium Psychrobacillus sp. strain AK 1817 was isolated from a tropical soil sample collected in Taiwan. Strain AK 1817 biotransforms the ergostane triterpenoid antcin K from the fungus Antrodia cinnamomea. The genome was sequenced using the PacBio RS II platform and consists of one chromosome of 4,096,020 bp, comprising 3,907 protein-coding genes, 75 tRNAs, 30 rRNAs, 5 noncoding RNAs (ncRNAs), and 100 pseudogenes.
Highlights
Psychrobacillus sp. strain AK 1817 is a Gram-positive, motile, endospore-forming, rod-shaped bacterium; of 4,311 soil bacteria isolated using the plating method, only Psychrobacillus sp. strain AK1817 was able to biotransform the triterpenoid antcin K into antcamphin E and antcamphin F (1)
The reads were assembled de novo using Canu v1.6 (2) with default settings
From the phylogenetic tree (Fig. 1), one can see that AK 1817 was closest to Psychrobacillus sp. strain BL-248-WT-3 (GenBank accession number NZ_JABAFC000000000), which was identified from domestic pig feces
Summary
Psychrobacillus sp. strain AK 1817 is a Gram-positive, motile, endospore-forming, rod-shaped bacterium; of 4,311 soil bacteria isolated using the plating method, only Psychrobacillus sp. strain AK1817 was able to biotransform the triterpenoid antcin K into antcamphin E and antcamphin F (1). Sequencing generated 149,490 subreads with a total length of 1,232,710,752 bp for a genome coverage of ;300Â. 149,490 subreads were obtained, for which the average, N50, and maximum read lengths were 8,246, 11,029, and 50,776 bp, respectively. The reads were assembled de novo using Canu v1.6 (2) with default settings.
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