Complete Genome Sequence of the Lytic Giant Bacteriophage pT24 Infecting Tenacibaculum spp., Isolated from a Shrimp Culture Pond.

Ho Viet Khoa,Goshi Kato,Matthura Labaiden,Ikuo Hirono,Tsubasa Uchino,Hidehiro Kondo,Sataporn Direkbusarakom,Toshihiro Nakai,Motohiko Sano,Yuki Midorikawa

doi:10.1128/genomea.00081-17

Abstract

ABSTRACTThe lytic bacteriophage pT24, which infects Tenacibaculum spp., was isolated from the water of a whiteleg shrimp (Litopenaeus vannamei) culture pond in Thailand. This giant bacteriophage with myovirus morphology comprised 234,670 bp with 296 predicted genes.

Highlights

The lytic bacteriophage pT24, which infects Tenacibaculum spp., was isolated from the water of a whiteleg shrimp (Litopenaeus vannamei) culture pond in Thailand
DNA of the phage was sequenced with the Illumina MiSeq platform using the Nextera XT library preparation kit and the MiSeq version 3 reagent kit (600 cycles)
The sequence reads were imported into CLC Genomics Workbench version 9 (CLC bio) and de novo assembled

Summary

Introduction

The lytic bacteriophage pT24, which infects Tenacibaculum spp., was isolated from the water of a whiteleg shrimp (Litopenaeus vannamei) culture pond in Thailand. DNA of the phage was sequenced with the Illumina MiSeq platform using the Nextera XT library preparation kit and the MiSeq version 3 reagent kit (600 cycles). The sequence reads were imported into CLC Genomics Workbench version 9 (CLC bio) and de novo assembled.

Results

Conclusion