Abstract
ABSTRACTRhodotorula kratochvilovae strain LS11 is a biocontrol agent (BCA) selected for its antagonistic activity against several plant pathogens both in the field and postharvest. Genome assembly includes 62 contigs for a total of 22.56 Mbp and a G+C content of 66.6%. Genome annotation predicts 7,642 protein-encoding genes.
Highlights
Rhodotorula kratochvilovae strain LS11 is a biocontrol agent (BCA) selected for its antagonistic activity against several plant pathogens both in the field and postharvest
Genome annotation was performed using the software Augustus trained with model genes found by BUSCO in the assembly, and this first prediction was used as input for MAKER together with 8,294 proteins of Rhodosporidium toruloides available in GenBank [27,28,29]
The availability of the genome sequence of R. kratochvilovae LS11 coupled with tools for genetic manipulation that we developed [30] represents a crucial step toward the understanding of the molecular mechanisms behind the biocontrol activity of this yeast and its ability to degrade the mycotoxin patulin
Summary
Rhodotorula kratochvilovae strain LS11 is a biocontrol agent (BCA) selected for its antagonistic activity against several plant pathogens both in the field and postharvest. Whole-genome sequencing was performed by Macrogen using PacBio sequencing technology, with a starting data set of ϳ150,000 reads ranging from 35 bp to 43,052 bp for a total of 837 million sequenced bases.
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