Abstract
Many vectors that are commonly used in the baculovirus/insect cell system (BICS) are derived from the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) strain E2. To facilitate work with these vectors, we sequenced the E2 genome, compared it to that of the AcMNPV C6 strain, and found that they are very similar overall.
Highlights
Many vectors that are commonly used in the baculovirus/insect cell system (BICS) are derived from the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) strain E2
Baculoviral vectors used in the BICS are almost always derived from Autographa californica multiple nucleopolyhedrovirus (AcMNPV) strains C6 or E2
The AcMNPV C6 genome sequence was reported in 1994 [11], which provided valuable information for the production and screening of baculoviral vectors derived from this strain
Summary
Many vectors that are commonly used in the baculovirus/insect cell system (BICS) are derived from the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) strain E2. Baculoviral vectors used in the BICS are almost always derived from Autographa californica multiple nucleopolyhedrovirus (AcMNPV) strains C6 (vector including BacPAK/flashBAC, Bac-N-Blue, and BaculoGold) or E2 (vectors including BestBac, BaculoDirect [9], Bac-to-Bac [10], and MultiBac). The AcMNPV C6 genome sequence was reported in 1994 [11], which provided valuable information for the production and screening of baculoviral vectors derived from this strain. Only partial sequences are available for AcMNPV E2.
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