Complete Genome Sequence of Paenibacillus strain Y4.12MC10, a Novel Paenibacillus lautus strain Isolated from Obsidian Hot Spring in Yellowstone National Park.

David A Mead,Roxanne Tapia,Catherine Brumm,John C Detter,Miriam Land,Susan Lucas,Thomas Schoenfeld,Rebecca Hochstein,Sam Pitluck,Alla Lapidus,Alex Copeland,Xiaojing Zhang,Tanja Woyke,Olga Chertkov,Galina Ovchinnikova,Nikos C Kyrpides,Natalia Ivanova ,Loren Hauser ,David Bruce ,Cliff Han ,Jan‐Fang Cheng ,Yun Juan Chang ,Lynne Goodwin ,Phillip J Brumm

doi:10.4056/sigs.2605792

Abstract

Paenibacillus sp.Y412MC10 was one of a number of organisms isolated from Obsidian Hot Spring, Yellowstone National Park, Montana, USA under permit from the National Park Service. The isolate was initially classified as a Geobacillus sp. Y412MC10 based on its isolation conditions and similarity to other organisms isolated from hot springs at Yellowstone National Park. Comparison of 16 S rRNA sequences within the Bacillales indicated that Geobacillus sp.Y412MC10 clustered with Paenibacillus species, and the organism was most closely related to Paenibacillus lautus. Lucigen Corp. prepared genomic DNA and the genome was sequenced, assembled, and annotated by the DOE Joint Genome Institute. The genome sequence was deposited at the NCBI in October 2009 (NC_013406). The genome of Paenibacillus sp. Y412MC10 consists of one circular chromosome of 7,121,665 bp with an average G+C content of 51.2%. Comparison to other Paenibacillus species shows the organism lacks nitrogen fixation, antibiotic production and social interaction genes reported in other paenibacilli. The Y412MC10 genome shows a high level of synteny and homology to the draft sequence of Paenibacillus sp. HGF5, an organism from the Human Microbiome Project (HMP) Reference Genomes. This, combined with genomic CAZyme analysis, suggests an intestinal, rather than environmental origin for Y412MC10.

Highlights

Numerous novel microorganisms have been isolated from hot springs in Yellowstone National Park
The bacterial isolate Y412MC10 was one of four microorganisms isolated from Obsidian Hot Spring, Yellowstone National Park, Montana, USA and submitted for whole genome sequencing
The analysis was carried out using only type strains of validly-named organisms, and the analysis shows that Y412MC10 does not clade with known Geobacillus species

Summary

Introduction

Numerous novel microorganisms have been isolated from hot springs in Yellowstone National Park. The bacterial isolate Y412MC10 was one of four microorganisms isolated from Obsidian Hot Spring, Yellowstone National Park, Montana, USA and submitted for whole genome sequencing. Following assembly of the complete genome of Y412MC10, the 16S rRNA sequence and genome properties properly assigned the organism as a Paenibacillus sp. Isolated from a hot spring to have its genome completely sequenced.

Results

Conclusion