Abstract
We report the complete genome sequence of a colistin-resistant strain of uropathogenic Escherichia coli, isolated in January 2013 at King Abdulaziz Medical City (KAMC), Riyadh, Saudi Arabia. The isolate (named SA186) was sequence type 131 (ST131) and belonged to serotype O25b-H4 and clade B (fimH22).
Highlights
The isolate was grown on Columbia agar (Oxoid) at 37°C, and genomic DNA was extracted from an overnight single starting colony culture using the QIAsymphony SP instrument following the manufacturer’s instructions (Qiagen)
The genome of SA186 consisted of one 4,828,837-bp chromosome with a GC content of 50.68% and five plasmids designated pSA186-MCR-1 (241,600 bp, 46.24% GC content), pSA186-2 (198,748 bp, 49.36% GC content), pSA186-3 (113,162 bp, 50.66% GC content), pSA186-4 (96,658 bp, 48.11% GC content), and pSA186-5 (106.936 bp, 48.88% GC content)
KP347127) [1], which were recovered from human and animal E. coli isolates belonging to ST602 (Switzerland), ST362 (Germany), and an undetermined ST (China), respectively. This is the first report of a complete genome sequence of a multiresistant sequence type 131 (ST131) fimH22 subclone non-extended-spectrum -lactamase/carbapenemaseproducing uropathogenic Escherichia coli (UPEC) strain from Saudi Arabia, which harbors the mcr-1 gene
Summary
The isolate was grown on Columbia agar (Oxoid) at 37°C, and genomic DNA was extracted from an overnight single starting colony culture using the QIAsymphony SP instrument following the manufacturer’s instructions (Qiagen). The complete genome sequence of SA186 was determined using the PacBio RS II singlemolecule real-time (SMRT) instrument at the Centre for Genomic Research at the University of Liverpool (United Kingdom). PacBio sequence reads were assembled following the Hierarchical Genome Assem-Volume 8 Issue 18 e00104-19
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