Phenotypic and functional characterization of mesenchymal stem cells from decidua parietalis of human term placenta

Abstract

s / Placenta 36 (2015) A1eA60 A28 3660, Mail Code 3124, Riyadh 11481, Saudi Arabia; King Abdullah International Medical Research Center, Riyadh, 11481, Saudi Arabia; King Saud Bin Abdulaziz University for Health Sciences, College of Applied Medical Sciences, Riyadh, 11481, Saudi Arabia; King Abdulaziz Medical City, Department of Pathology, Riyadh, 11481, Saudi Arabia; University of Melbourne, Department of Obstetrics and Gynaecology and Department of Perinatal Medicine Pregnancy Research Centre, Royal Women’s Hospital, Parkville, Victoria, Australia Mesenchymal stem cells isolated from the decidua parietalis of the human term placenta (DPMSCs) have been reported as promising cells for use in regenerative medicine. However, the characteristics of DPMSCs are not fully elucidated and therefore, this study was conducted to isolate and characterize DPMSCs to reveal their potential use in stem cell-based therapy. DPMSCs were isolated from the decidua parietalis using the enzymatic digestion method and then were phenotypically characterized by flow cytometry and were differentiated into adipocytes, osteocytes and chondrocytes using differentiation media as demonstrated by cytochemical staining. The expression profiles of genes and proteins of DPMSCs were also characterized using real time polymerase chain reaction (PCR) and flow cytometry, respectively. In addition, the secretion of cytokines by DPMSCs was also evaluated using the sandwich enzyme-linked immunosorbent assay (ELISA) technique. Moreover, DPMSCs were exposed to inflammatory stimuli to determine their proliferation and migration abilities. MSCs were isolated from the decidua parietalis of human term placenta and these DPMSCs expressed CD44, CD90, CD105, CD146, CD166 and HLA-ABC but not CD14, CD19, CD40, CD45, CD80, CD83, CD86 and HLA-DR. In addition, these DBMSCs differentiated into osteocytes, chondrocytes and adipocytes and they also expressed a broad spectrum of adhesion molecules, chemokines and their receptors, growth factor receptors and cytokines and their receptors. Moreover, they secreted inflammatory and anti-inflammatory cytokines and they were able to proliferate and migrate in response to an inflammatory environment. In this study, we have shown that DPMSCs express a broad spectrum of biological factors which are possibly employed by these cells to mediate their cellular functions of adhesion, migration, homing, immune modulation and angiogenesis. Therefore, we propose that MSCs isolated from the decidua parietalis of human term placenta as an attractive source of MSCs for stem cell therapy. P1.51. PHENOTYPIC AND FUNCTIONAL CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM DECIDUA BASILS OF HUMAN TERM PLACENTA Mohamed Abumaree , Fawaz Abomaray , Khaled Al Saad , Dunia Jawdat , Abdulaziz Al Khaldi , Ahmed Al Askar , Seham Al Harthy , Abdulmohesen Alkushi , Bill Kalionis , Mohammed Al Jumah . King Saud Bin Abdulaziz University for Health Sciences, College of Science and Health Professions, Riyadh, 11426, Saudi Arabia; King Abdullah International Medical Research Center, Riyadh, 11426, Saudi Arabia; King Abdulaziz Medical City, Department of Pathology, Riyadh, 11426, Saudi Arabia; King Abdulaziz Medical City, Division of Cardiac Surgery, Riyadh, 11426, Saudi Arabia; King Abdulaziz City for Science and Technology, Riyadh, 11442, Saudi Arabia; University of Melbourne, Department of Obstetrics and Gynaecology and Department of Perinatal Medicine Pregnancy Research Centre, Royal Women’s Hospital, Parkville,