Abstract
Enterovirus B83 (EV-B83) is a recently identified member of enterovirus species B. It is a rarely reported serotype and up to date, only the complete genome sequence of the prototype strain from the United States is available. In this study, we describe the complete genomic characterization of an EV-B83 strain 246/YN/CHN/08HC isolated from a healthy child living in border region of Yunnan Province, China in 2008. Compared with the prototype strain, it had 79.6% similarity in the complete genome and 78.9% similarity in the VP1 coding region, reflecting the great genetic divergence among them. VP1-coding region alignment revealed it had 77.2–91.3% with other EV-B83 sequences available in GenBank. Similarity plot analysis revealed it had higher identity with several other EV-B serotypes than the EV-B83 prototype strain in the P2 and P3 coding region, suggesting multiple recombination events might have occurred. The great genetic divergence with previously isolated strains and the extremely rare isolation suggest this serotype has circulated at a low epidemic strength for many years. This is the first report of complete genome of EV-B83 in China.
Highlights
Human enteroviruses (EVs), comprising more than 100 serotypes, belong to the genus Enterovirus, family Picornaviridae[1]
The human enteroviruses are classified into four species: Enterovirus A (EV-A), EV-B, EV-C, and EV-D3
In a previous study[12], we reported the identification of two Enterovirus B83 (EV-B83) strains isolated in Yunnan Province of China in 1999 via sequencing the partial VP1 region
Summary
Human enteroviruses (EVs), comprising more than 100 serotypes, belong to the genus Enterovirus, family Picornaviridae[1]. They are among the most common human viruses, infecting an estimated number of a billion people annually worldwide[2]. There were several decades where new enteroviruses were uncommonly identified until the introduction of molecular detection methods in 19995,6 In this system, members within an EV serotype share greater than 75% nucleotide (85% amino acid) identity in the VP1 coding region. Based on the AFP surveillance, several newer EV types have been identified and the complete genome has been sequenced and analyzed in China[15,16,17,18,19]
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