Abstract
The complete chloroplast genome of Wonwhang (BioSample SAMN05196235), Pyrus pyrifolia, was assembled and analyzed by de novo assembly using whole-genome sequencing data. The accession NC_015996 was used as a reference sequence in this study. The total chloroplast genome size of the Wonwhang was 159,922 bp in length, including a pair of inverted repeat regions (IRs) of 26,392 bp that are separated by a large single-copy region of 72,023 bp and a small single-copy region of 19,235 bp. A total of 132 genes, including 93 protein-coding genes, 31 tRNA genes and eight rRNA genes, were predicted from the chloroplast genomes. Among them, 18 genes occur in IRs, containing nine protein-coding genes, five tRNA genes and four rRNA genes. The GC content of Wonwhang chloroplast genome is 36.6%. The phylogenetic analysis with nine Rosids species and three other species revealed that Wonwhang was clustered with Malus genus.
Highlights
Pears belong to the family of Rosaceae and have been cultivated in East Asia, Europe and North America for more than 3000 years, and are among the most important fruit crops in temperate regions (Bell 1990)
We sequenced the complete chloroplast genomes of Wonwhang based on an Illumina platform
The plant sample of Wonwhang was collected from Naju, Korea, and representatively identified by Dr YK Kim of the Pear Research Station, National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA)
Summary
Pears belong to the family of Rosaceae and have been cultivated in East Asia, Europe and North America for more than 3000 years, and are among the most important fruit crops in temperate regions (Bell 1990). KEYWORDS Pyrus pyrifolia; Wonwhang; chloroplast genome; phylogenetic analysis; whole-genome sequencing In South Korea, pear breeding began in the late 1920s by National Institute of Horticultural and Herbal Science (NIHHS) of Rural Development Administration (RDA). To improve the fruit quality in terms of fruit size, sugar content, flesh firmness and storage quality, many breeders frequently used interspecific hybridization (Kim 2016).
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