Compatibility studies of indigenous Trichoderma isolates with pesticides

Abstract

A total of 40 Trichoderma isolates collected from North-Eastern dry zone of Karnataka. Isolates were identified at species level by sequencing of tef1 gene. The gene sequencing results revealed that T. asperellum and Hypocrea lixii were predominantly distributed throughout the surveyed area. Among the isolates sequenced, 27 represents as T. asperellum and nine as Hypocrea lixii, two as T. harzianum and each one as T. afroharzianum and T. asperelloides. Trichoderma isolates expressed the pertinent compatibility with pesticides tested, Carbendazim and Hexaconazole fungicides were not compatible at any given concentration with the isolates. However, maximum compatibility was found in T-45, T-2, T-9 and T-16 isolates against Mancozeb at 0.15 and 0.20 per cent. The recommended dose (RD) of Chlorpyrifos (0.15 %), Imidacloprid (0.03 %) and Acephate (0.1 %) insecticide was found highly compatible with all the isolates. The RD of herbicide 2, 4-D and Glyphosate are more compatible than the Pendimethalin. Among the agrochemicals tested Mancozeb; Chlorpyrifos; Imidacloprid; Acephate; 2, 4-D and Glyphosate are compatible with Trichoderma isolates. The T-45, T-42, T-39 and T-43 isolates were identified as best pesticide tolerant isolates. Incorporation of pesticide tolerant Trichoderma strains in integrated diseases management (IDM) provides added advantages in many perspectives.