Comparitive evaluation of antimicrobial efficacy of 0.1% octenidine dihydrochloride, 2% chlorhexidine and 2% chitosan against E.faecalis within the dentinal tubules

Abstract

Root canal treatment is a type of endodontic therapy used to perform for the elimination of the micro-organisms by standardized mechanical instrumentation and adequate cleaning and shaping of the infected root canal. The readily available root canal irrigants which are used in endodontic therapy have shown somewhat toxic and harmful side effects when used at certain concentration, some of the endodontic irrigants have shown species specific resistance to a particular microbial load that’s why there is a need to discover newer irrigants which are non toxic, effective and can be used safe to use.In vitro evaluation and comparision of the antimicrobial efficacy of 0.1% octenidine dihydrochloride (OCT), of 2% chlorhexidene (CHX) and 2% chitosan as root canal irrigant against E. faecalis within the dentinal tubules at 200µ and 400µm depth.Seventy two freshly extracted mandibular molars were decoronated and the mesial root specimen was standardized till working length. E.faecalis (strain MTCC 439) was grown on brain heart infusion sheep blood agar plate until seven days and the decoronated root specimens collected were divided into four groups (n=18) based on irrigation protocol: Group 1 – 2% chlorhexidene (CHX), Group 2 - 0.1% octenidine dihydrochloride (OCT), Group 3- 2% chitosan and Group 4 – normal saline . Each specimen was irrigated with particular irrigant till 3 minutes. Dentin shavings were obtained from root specimens using diamond disc and these dental chips were used to calculate the colony forming unit at 200µ and 400µm depth. The data obtained after the experiment was statistically analyzed.Non significant difference has been shown by group 1when compared with group 2 while significant difference has been shown by group 3 when compared with group 1 and group 2 by taking into account that group 4 is taken as control group. Significant difference was found when all the groups 1-4 were compared at 200 and 400 µm depth.It was observed and evaluated that antimicrobial efficacy of 2% chitosan against e. faecalis was found to be higher as compred to 2% chlorhexidene (CHX) and 0.1% octenidine dihydrochloride (OCT) both at 200µ and 400µm depth.