Abstract
Objective To investigate the effects of lights with different wavelength on the retina of rd12 and C57BL/6J mice.Methods Thirty two rd12 mice and C57BL/6J mice were randomly divided into the control group,white light group,mid-wavelength light (505 nm) group and short-wavelength light (405nm) group,with eight mice in each group.Besides the control group,other groups were exposed to cycle illuminations [12 hours dark,12 hours (800 ± 130) Lux] for seven days to establish the model of retinal light damage.Electroretinogram (ERG) responses of all mice were recorded at the day before illumination and 1st,4th and 7th days after illumination.The eyes were enucleated at 7th days after illumination to assess levels of reactive oxygen species (ROS),expression of peroxiredoxin 6 (PRDX6),and activity of caspase-3.Results ERG amplitudes of all groups declined gradually in C57BL/6J mice,and the most significant effects was found in the short-wavelength light group.The amplitudes of photopic b-wave were significantly different at 1st,4th and 7th days (F=4.412,5.082,9.980; P＜0.01).The amplitudes of cone b-wave of the four groups decreased to (85±10) ％,(70±19) ％,(57±22) ％ and (46±19) ％ at 7th days,respectively,and were significantly different between white light group and short-wavelength light group(t=3.19,P＜0.01).The levels of ROS were significantly different in rd12 mice (F=16.08,P＜0.01),and elevated obviously in short-wavelength light group.The expressions of PRDX6 of retina were significantly different in rd12 mice (F =7.214,P＜0.05),and were decreased obviously in shortwavelength light group.The caspase-3 relative activity was significantly different in rdl2 retina (F=7.530,P＜0.05) ; but there was no significant difference in C57BL/6J mice (F=3.625,1.993,1.133; P＞0.05).The caspase-3 relative activity were significant different between rd12 mice and C57BL/6J mice in short wavelength light group (t =5.474,P＜0.05).Conclusions Short-waveleugth light can induce retinal damage of mouse retina,especially in rd12 mouse.The retinal light damage possibly relates to the oxidative damage. Key words: Photoc stimulation/adverse effects; Electroretinography; Comparative study; Animal experimentation
Published Version
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