Abstract
Lasers began to be used in the treatment of infertility between 1980s-1990; the first studies clearly indicate that human sperm motility as well as velocity can be improved by He-Ne laser irradiation. After collection, the semen was taken immediately to the laboratory for sample preparation and physical and morphological analysis. Semen samples were stored in formalin-saline for evaluation of concentration and sperm morphology. The samples of semen used were of concentration greater than 200 × 106/mL, and ejaculate volume greater than 5.0 ml. The gathered data suggest that primary photo acceptors are connected with oxygen metabolism and, in particular, with respiratory chains. It is important to recall that the activation by using (He-Ne)laser more effective than that when we used diode laser, because the activation by red light more effective to activate the ATP production ,and as a result increase sperm mutuality red laser light energized the sperm more than the invisible light absorption. The photo receptors when using red light were more sensitive to the red light than the invisible (Diode laser).
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