Comparison of Types and Amounts of Nanoscale Heterogeneity on Bacteria Retention

Scott A Bradford,Salini Sasidharan,Gukhwa Hwang,Hyunjung Kim

doi:10.3389/fenvs.2018.00056

Abstract

Interaction energy calculations that assume smooth and chemically homogeneous surfaces are commonly conducted to explain bacteria retention on solid surfaces, but experiments frequently exhibit signification deviations from these predictions. A potential explanation for these inconsistencies is the ubiquitous presence of nanoscale roughness (NR) and chemical heterogeneity (CH) arising from spatial variability in charge (CH1), Hamaker constant (CH2), and contact angles (CH3) on these surfaces. We present a method to determine the mean interaction energy between a colloid and a solid-water-interface (SWI) when both surfaces contained binary NR and CH. This approach accounts for double layer, van der Waals, Lewis acid-base, and Born interactions. We investigate the influence of NR and CH parameters and solution ionic strength (IS) on interaction energy profiles between hydrophilic and hydrophobic bacteria and the SWI. Increases in CH1 and CH3 reduce the energy barrier and create deeper primary minima on net electrostatically unfavorable surfaces, whereas increasing CH2 diminishes the contribution of the van der Waals interaction in comparison to quartz and makes a more repulsive surface. However, these roles of CH are always greatest on smooth surfaces with larger fractions of CH. In general, increasing CH1 and CH3 have a larger influence on bacteria retention under lower IS conditions, whereas the influence of increasing CH2 is more apparent under higher IS conditions. However, interaction energy profiles are mainly dominated by small fractions of NR, which dramatically lower the energy barrier height and the depths of both the secondary and primary minima. This significantly increases the relative importance of primary to secondary minima interactions on net electrostatically unfavorable surfaces, especially for conditions that produce small energy barriers on smooth surfaces. Energy balance calculations indicate that this primary minimum is sometimes susceptible to diffusive removal depending on the NR and CH parameters.

Highlights

Colloids are particles with diameters of around 10 nm to 10 μm and include microorganisms, dissolved and particulate organic matter, clays and mineral precipitates, and nanoparticles (DeNovio et al, 2004)
The solid phase Hamaker constant and fraction on site 2 were varied from As2 = 2.5 × 10−20 to 10 × 10−20 J and fs2 = 0 to 1 when considering heterogeneity in van der Waals interactions
These changes were not sufficient to eliminate the energy barrier because the influence of the repulsive electrostatic double layer interaction is greater under lower ionic strength (IS) conditions

Summary

Introduction

Colloids are particles with diameters of around 10 nm to 10 μm and include microorganisms, dissolved and particulate organic matter, clays and mineral precipitates, and nanoparticles (DeNovio et al, 2004). The adhesive force is typically determined from interaction energy calculations (Bergendahl and Grasso, 1999). Many experimental observations of colloid retention and release have not been consistent with such interaction energy calculations (Suresh and Walz, 1996; Huang et al, 2009; Bendersky and Davis, 2011). A number of researchers have extended interaction energy calculations to include NR and/or CH as a means to explain colloid retention and release (e.g., Suresh and Walz, 1996; Bhattacharjee et al, 1998; Hoek et al, 2003; Hoek and Agarwal, 2006; Huang et al, 2009; Bendersky and Davis, 2011; Henry et al, 2011)

Methods

Results

Conclusion