Abstract
Objective: To compare Transforming growth factor-β1 (TGF-β1) expression in various L-PRF concentrations on the hDPSC differentiation process. Material and Methods: hDPSC cell cultures were subjected to serum starvation by reducing FBS levels in the hDPSC culture media. Lysate PRF was obtained from the PRF gel, which was then incubated at 4°C for 24 h. The supernatant was dried, transferred to a 2-ml Eppendorf tube, and stored at −20°C. The evaluation of TGF-β1 expression in 1%, 5%, 10%, and 25% L-PRF samples and 10% FBS (control) during the process of hDPSC differentiation was quantified using an ELISA reader on day 7. The expression of TGF-β1 was subjected to a one-way ANOVA test, followed by Bonferroni’s post hoc test with significant values (p<0.05). Results: Significant differences were noted in TGF-β1 expression between 1%, 5%, 10%, and 25% L-PRF and the control group (10% FBS). The highest TGF-β1 expression occurred with 25% L-PRF (0.645 ± 0.048), followed by 10% L-PRF (0.461 ± 0.035), 10% FBS (0.374 ± 0.013), 5% L-PRF (0.275 ± 0.045), and the lowest expression was with 1% L-PRF (0.160 ± 0.045). Conclusion: The best result of TGF-B1 expression in hDPSC differentiation was in the 25% L-PRF group.
Highlights
Regenerative endodontic treatment (RET) has led to a paradigm shift in endodontic treatment through pulp regeneration
Transforming growth factor-β1 (TGF-β1) can initiate the differentiation of human dental pulp stem cells into several other cell types through limited lineages or multipotent, such as odontoblasts
There were no significant differences between the control group (10% FBS) and the groups with 5% and 10% L-PRF
Summary
Regenerative endodontic treatment (RET) has led to a paradigm shift in endodontic treatment through pulp regeneration. It was reported that RET was based on the basic principles of tissue engineering, which involves three main components: stem cells, a scaffold comprising an extracellular matrix framework, and growth factors as signaling proteins [1,2,3]. Transforming growth factor-β1 (TGF-β1) is a polypeptide member of the beta cytokine transformation superfamily. This protein is secreted to perform many cellular functions, including cell growth, proliferation, differentiation, senescence, and apoptosis [4]. TGF-β1 can initiate the differentiation of human dental pulp stem cells (hDPSCs) into several other cell types through limited lineages or multipotent, such as odontoblasts. TGF-β plays an important role in reducing the number of DNA (Id) binding protein inhibitors, known as inhibiting factors for protein differentiation. TGF β1 regulates the transition process, from proliferation to differentiation, so that the process will be stopped and continue to differentiation process [5]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
