Abstract
To determine whether aqueous and ethanol fractions of the Angelica keiskei leaf exert toxicity when used for cosmetic purposes, we performed the acute eye irritancy test. Animals were treated with sample fractions (100 mg/dose) according to standard procedure guidelines. No significant changes or damage was detected in the fraction-treated groups in terms of ocular lesions in the cornea, the size of the cornea with turbidity, swelling of the eyelid and emission discharge. However, sodium dioctyl sulfosuccinate, a positive control, induced severe toxic symptoms. Thus, aqueous and ethanol fractions of Angelica keiskei do not appear to induce acute toxicity in the eye lens, as assessed from anatomical and pathological observations in the rabbit eye. Our results collectively suggest that aqueous and ethanol fractions show promise as cosmetic ingredients that do not cause eye toxicity.
Highlights
Many medicinal plants provide beneficial sources of vitamins, dietary fiber and phytochemicals
Mice sensitized to ovalbumin were orally administered the Angelica archangelica extract and their lungs were analyzed via hematoxylin and eosin (H&E) staining to measure the IL-4/13 cytokine content
Following instillation of the aqueous and ethanol fractions of the Angelica keiskei leaf, eye lens mucosa was evaluated for local mucosal irritation
Summary
Various adverse effects including acute/chronic toxicity, irritation and sensitization, can be assessed using in vivo, in vitro, semi in vivo and ex vivo animal models, even in modified tests [11,12,13]. A specific component or constituent should not exert toxic effects on the skin ( the eye in the case of cosmetics) and should only be passed and approved in cases where no damage/changes to the eye lens are observed in animals or clinical trials for the development of cosmetics [14]. SON et al: EVALUATION OF Angelica keiskei LEAF FRACTIONS IN THE EYE IRRITANCY TEST. We performed the acute eye lens mucosal irritation test with Angelica keiskei leaf fractions using an in vivo animal model.
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