Abstract

Previous studies in several laboratories have demonstrated marked species differences in the hormonal responsiveness of mammalian adipose tissue. The present investigation extends these comparative studies by comparing the antilipolytic potency of insulin upon the perirenal adipose tissue of rat, hamster, guinea pig and rabbit. Epididymal adipose tissue of the rat was also studied. Lipolysis was stimulated in vitro in slices of adipose tissue from these 4 species by incubation with 1 μg/ml epinephrine, adrenocorticotropin (ACTH) or β-melanocyte-stimulating hormone (β-MSH) in albumin-free, glucosefree Krebs-Ringer phosphate medium; the lipolytic response to these hormones, in the absence and presence of 0.00001-10 μg/ml insulin, was determined by measuring the free fatty acid (FFA) concentration in the tissue slices after a 2-hr incubation. Bovine insulin at a concentration of 0.0001-.001 μg/ml or greater inhibited the lipolytic response of rat adipose tissue to epinephrine or ACTH by 50-95 %. At a concent...

Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call