Abstract
Senecavirus A (SVA), an emerging infectious disease, is associated with the porcine idiopathic vesicular disease. Here, the pathogenesis of different strains of SVA was investigated in growing-finishing pigs. We aimed to evaluate the replication characteristics, virus particle morphology, clinical signs, and vesicular lesions in comparison with two different strains of SVA. The animals were infected with SVA HB-CH-2016 or CH/AH-02/2017 by intranasal routes (3 mL, 109TCID50/mL) and monitored daily for 14 days post-inoculation (dpi) for clinical signs and vesicular lesions. Viremia or viral shedding was detected in the blood, fecal swab, and nasal swab samples. Results showed no distinct differences in plaque size, replication ability, and characteristic virions between SVA HB-CH-2016 and CH/AH-02/2017 strains. Animal experimental results showed that both SVA CH/AH-02/2017 and SVA HB-CH-2016 could infect pigs. However, an obvious difference in the pathogenicity and dynamics of infection was observed between SVA HB-CH-2016 and CH/AH-02/2017 strains. The pathogenesis of SVA CH/AH-02/2017 was similar to that of published results of USA strains, whereas the SVA HB-CH-2016 strain had low pathogenicity to pigs. Clinical signs and vesicular lesions were observed in SVA CH/AH-02/2017-infected pigs. Additionally, the different branches of SVA should be capable of inducing broad cross-reactive neutralizing antibodies, which play an important role in clearing the SVA virus. This study of animal models for SVA infection will be beneficial to develop vaccines and antivirals.
Highlights
Senecavirus A (SVA), called the Seneca valley virus, is a member of the genus Senecavirus from the family Picornaviridae [1]
The analysis of the complete genomic sequence showed that SVA HB-CH-2016 shared 96% sequence identity with SVA CH/AH-02/2017
Phylogenetic analysis indicated that SVA HB-CH-2016 belonged to Canada-like strains, and SVA CH/AH-02/2017 belonged to USA-like SVA strains (Figure 1)
Summary
Senecavirus A (SVA), called the Seneca valley virus, is a member of the genus Senecavirus from the family Picornaviridae [1]. The virus is a positive, single-strand RNA virus. The genome of SVA is approximately 7.2 kb in length, which contains a single open reading frame (ORF). The ORF encoding a polyprotein of 2181 amino acids, consists of 12 proteins (Lpro, VP1, VP2, VP3, VP4, 2A, 2B, 2C, 3A, 3B, 3C, and 3D) [1,2,3]. Structural biology analysis revealed that the B-cell epitopes of SVA mainly exist in VP2 and VP3 proteins (in our laboratory). The VP2 and VP3 proteins are important targets for designing gene engineering vaccines and diagnostic agents
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
