Isolation and full-genome sequencing of Seneca Valley virus in piglets from China, 2016.

Suhong Qian,Huanchun Chen,Wenchun Fan,Xiangmin Li,Ping Qian

doi:10.1186/s12985-016-0631-2

Abstract

BackgroundSeneca valley virus (SVV), a member of the Picornaviridae family, is a small non-enveloped RNA virus, that is linked to porcine idiopathic vesicular disease (PIVD). SVV infection in swine results in vesicular disease and epidemic transient neonatal losses (ETNL). The first case of SVV infection was reported in Guangdong, South China in 2015.ResultsWe isolated and characterized an SVV HB-CH-2016 strain from vesicular lesion tissue specimens from piglets with PIVD in Hubei, Central China. The complete genome sequence of SVV HB-CH-2016 strain shares high nucleotide identities (94 to 99 %) with all previously reported SVV genomes, moreover, the polyprotein accounts for 98–99 % of amino acid sequence identity. Therefore, the SVV HB-CH-2016 strain is closely related to the SVV CH-01-2015 strain.ConclusionsThe case reported in this paper is the second SVV infection case in China. Our findings demonstrate that sporadic SVV infection has occurred in Central China, and therefore, active surveillance on the swine population is important. Moreover, veterinarians must pay attention to this vesicular disease and reinforce biosecurity measures and prevent SVV spread.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-016-0631-2) contains supplementary material, which is available to authorized users.

Highlights

Seneca valley virus (SVV), a member of the Picornaviridae family, is a small non-enveloped RNA virus, that is linked to porcine idiopathic vesicular disease (PIVD)
542 bp 366 bp 298 bp Results We successfully isolated the infectious SVV HB-CH2016 strain from piglets with PIVD, which was negative for vesicular stomatitis virus (VSV), foot-and-mouth disease virus (FMDV) or swine vesicular disease virus (SVDV), but positive for SVV (Fig. 1)
Analysis of the full-genome sequence of SVV HB-CH-2016 strain indicated that it shares high nucleotide identities (94 to 99 %) with all previous SVV genomes in the GenBank (Fig. 3a), and the polyprotein shares 98–99 % amino acid sequence identity (Fig. 3b)

Summary

Results

We successfully isolated the infectious SVV HB-CH2016 strain from piglets with PIVD, which was negative for VSV, FMDV or SVDV, but positive for SVV (Fig. 1). The fourth passage SVV isolate induced typical cytopathic effects characterized by rounding and shrinkage and degeneration of BHK-21 cells at 18 h post-infection. Western blot analysis showed approximately 30 kilodalton (kDa) band in cells infected with the isolate (Fig. 2d, lane 1) but not in the mock cells (Fig. 2d, lane 2). Analysis of the full-genome sequence of SVV HB-CH-2016 strain indicated that it shares high nucleotide identities (94 to 99 %) with all previous SVV genomes in the GenBank (Fig. 3a), and the polyprotein shares 98–99 % amino acid sequence identity (Fig. 3b). Evolutionary dynamics, pathogenesis and epidemiological features of SVV infection, is crucial in facilitating the development of antiviral strategies and offering effective control measures against SVV infection

Conclusions

Background

Methods

Discussion