Comparison of the massive pulmonary embolism of the animal model stabled by different methods

Abstract

To contrast the massive pulmonary embolism of the animal model had been stabled by inserting the animal's own blood clots or through use of detachable latex balloons. Eighteen healthy sheep were separated as three groups with random. We established the animal lung, PE model by inserting the animal's own blood clots at right lung artery in 6 sheep, and 6 sheep were through used of detachable latex balloons to substitute the blood clots. Six healthy sheep were used as controls. Each sheep was anesthetized. Both sides of the region inguinalis were applied 21 transfixion pins to one femoral artery and two femoral veins on the left side, and one femoral vein on the right side. All vessels were inducted into the 5 F vagina vasorum except one vein for which 12 F was inducted. 50 ml blood was taken from vagina vasorum from each animal and stabilized for 2 h for later use as a body blood clot. Guided and visualized by X-ray, a 5 F pigtail catheter was inserted on the left side to measure arterial pressure, central venous pressure and pulmonary arterial pressure; a 12 F guiding catheter was then inserted through right side femoral vein into the right lung artery to inject 8 ml 350 mg I ultravist at 6 ml/s for pulmonary arteriography, After the animal was stabilized for 30 min, we input their own body blood clots (0.6 ml/kg) using a 12 F guiding catheter or, alternatively, to release a self-made, detachable latex balloon. We measured arterial pressure, central venous pressure, pulmonary arterial pressure, heart rate, blood oxygen saturation, partial pressure of oxygen in artery and partial pressure of carbon dioxide in artery etc. of the animals both immediately before inputting their own body blood clots or detachable latex balloons, immediately afterwards, immediately before interfering with airflow and at 3 min, 6 min, 10 min, 15 min, 20 min, 30 min, 45 min and 60 min after the interference. Heart and breath rates of all 12 sheep increased, blood oxygen saturation and partial pressure of oxygen in artery decreased, and pulmonary arterial pressure increased after inputting their own body blood clots or inserting latex balloons with statistical significance that were compared with controls. Blood oxygen saturation decreased, on average, by more than 25% within 30 min. Two hours after, all 12 experimental animals showed stable. Index of sheep in latex balloons group were increased slowly but stabilized at a lower level in blood clots group with statistical significance. This animal experiment demonstrates that balloon can establish a physico- affection and blood clot can establish a chemical action for massive PE.